Analysis of the SYSDIET Healthy Nordic Diet randomized trial based on metabolic profiling reveal beneficial effects on glucose metabolism and blood lipids
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Analysis of the SYSDIET Healthy Nordic Diet randomized trial based on metabolic profiling reveal beneficial effects on glucose metabolism and blood lipids. / Gürdeniz, Gözde; Uusitupa, Matti; Hermansen, Kjeld; Savolainen, Markku J; Schwab, Ursula; Kolehmainen, Marjukka; Brader, Lea Johanne; Cloetens, Lieselotte; Herzig, Karl-Heinz; Hukkanen, Janne; Rosqvist, Fredrik; Ulven, Stine Marie; Gunnarsdóttir, Ingibjörg; Thorsdottir, Inga; Orešič, Matej; Poutanen, Kaisa S; Risérus, Ulf; Åkesson, Björn; Dragsted, Lars Ove.
In: Clinical Nutrition, Vol. 41, No. 2, 2022, p. 441-451.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of the SYSDIET Healthy Nordic Diet randomized trial based on metabolic profiling reveal beneficial effects on glucose metabolism and blood lipids
AU - Gürdeniz, Gözde
AU - Uusitupa, Matti
AU - Hermansen, Kjeld
AU - Savolainen, Markku J
AU - Schwab, Ursula
AU - Kolehmainen, Marjukka
AU - Brader, Lea Johanne
AU - Cloetens, Lieselotte
AU - Herzig, Karl-Heinz
AU - Hukkanen, Janne
AU - Rosqvist, Fredrik
AU - Ulven, Stine Marie
AU - Gunnarsdóttir, Ingibjörg
AU - Thorsdottir, Inga
AU - Orešič, Matej
AU - Poutanen, Kaisa S
AU - Risérus, Ulf
AU - Åkesson, Björn
AU - Dragsted, Lars Ove
N1 - CURIS 2022 NEXS 020
PY - 2022
Y1 - 2022
N2 - Background & aims: Intake assessment in multicenter trials is challenging, yet important for accurate outcome evaluation. The present study aimed to characterize a multicenter randomized controlled trial with a healthy Nordic diet (HND) compared to a Control diet (CD) by plasma and urine metabolic profilesand to associate them with cardiometabolic markers. Methods: During 18e24 weeks of intervention, 200 participants with metabolic syndrome were advised at six centres to eat either HND (e.g. whole-grain products, berries, rapeseed oil, fish and low-fat dairy) or CD while being weight stable. Of these 166/159 completers delivered blood/urine samples. Metabolicprofiles of fasting plasma and 24 h pooled urine were analysed to identify characteristic diet-related patterns. Principal components analysis (PCA) scores (i.e. PC1 and PC2 scores) were used to test their combined effect on blood glucose response (primary endpoint), serum lipoproteins, triglycerides, andinflammatory markers.Results: The profiles distinguished HND and CD with AUC of 0.96 ± 0.03 and 0.93 ± 0.02 for plasma and urine, respectively, with limited heterogeneity between centers, reflecting markers of key foods. Markers of fish, whole grain and polyunsaturated lipids characterized HND, while CD was reflected by lipidscontaining palmitoleic acid. The PC1 scores of plasma metabolites characterizing the intervention is associated with HDL (β = 0.05; 95% CI: 0.02, 0.08; P = 0.001) and triglycerides (β = 0.06; 95% CI: -0.09, -0.03; P < 0.001). PC2 scores were related with glucose metabolism (2 h Glucose, β = 0.1; 95% CI: 0.05, 0.15; P < 0.001), LDL (β = 0.06; 95% CI: 0.01, 0.1; P = 0.02) and triglycerides (β 0.11; 95% CI: 0.06, 0.15; P < 0.001). For urine, the scores were related with LDL cholesterol. Conclusions: Plasma and urine metabolite profiles from SYSDIET reflected good compliance with dietary recommendations across the region. The scores of metabolites characterizing the diets associated with outcomes related with cardio-metabolic risk. Our analysis therefore offers a novel way to approach a per protocol analysis with a balanced compliance assessment in larger multicentre dietary trials.The study was registered at clinicaltrials.gov with NCT00992641.
AB - Background & aims: Intake assessment in multicenter trials is challenging, yet important for accurate outcome evaluation. The present study aimed to characterize a multicenter randomized controlled trial with a healthy Nordic diet (HND) compared to a Control diet (CD) by plasma and urine metabolic profilesand to associate them with cardiometabolic markers. Methods: During 18e24 weeks of intervention, 200 participants with metabolic syndrome were advised at six centres to eat either HND (e.g. whole-grain products, berries, rapeseed oil, fish and low-fat dairy) or CD while being weight stable. Of these 166/159 completers delivered blood/urine samples. Metabolicprofiles of fasting plasma and 24 h pooled urine were analysed to identify characteristic diet-related patterns. Principal components analysis (PCA) scores (i.e. PC1 and PC2 scores) were used to test their combined effect on blood glucose response (primary endpoint), serum lipoproteins, triglycerides, andinflammatory markers.Results: The profiles distinguished HND and CD with AUC of 0.96 ± 0.03 and 0.93 ± 0.02 for plasma and urine, respectively, with limited heterogeneity between centers, reflecting markers of key foods. Markers of fish, whole grain and polyunsaturated lipids characterized HND, while CD was reflected by lipidscontaining palmitoleic acid. The PC1 scores of plasma metabolites characterizing the intervention is associated with HDL (β = 0.05; 95% CI: 0.02, 0.08; P = 0.001) and triglycerides (β = 0.06; 95% CI: -0.09, -0.03; P < 0.001). PC2 scores were related with glucose metabolism (2 h Glucose, β = 0.1; 95% CI: 0.05, 0.15; P < 0.001), LDL (β = 0.06; 95% CI: 0.01, 0.1; P = 0.02) and triglycerides (β 0.11; 95% CI: 0.06, 0.15; P < 0.001). For urine, the scores were related with LDL cholesterol. Conclusions: Plasma and urine metabolite profiles from SYSDIET reflected good compliance with dietary recommendations across the region. The scores of metabolites characterizing the diets associated with outcomes related with cardio-metabolic risk. Our analysis therefore offers a novel way to approach a per protocol analysis with a balanced compliance assessment in larger multicentre dietary trials.The study was registered at clinicaltrials.gov with NCT00992641.
KW - Faculty of Science
KW - LC-MS metabolomics
KW - Healthy Nordic diet
KW - Randomized controlled trial
KW - Glucose and lipid metabolism
KW - Plasma metabolite scores
U2 - 10.1016/j.clnu.2021.12.031
DO - 10.1016/j.clnu.2021.12.031
M3 - Journal article
C2 - 35007813
VL - 41
SP - 441
EP - 451
JO - Clinical Nutrition
JF - Clinical Nutrition
SN - 0261-5614
IS - 2
ER -
ID: 289168317