Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae. / Aabo, Thomas Ask; Perch-Nielsen, Ivan R.; Dam, Jeppe Seidelin; Palima, Darwin; Siegumfeldt, Henrik; Glückstad, Jesper; Arneborg, Nils.
I: Journal of Biomedical Optics, Bind 15, Nr. 4, 2010.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae
AU - Aabo, Thomas Ask
AU - Perch-Nielsen, Ivan R.
AU - Dam, Jeppe Seidelin
AU - Palima, Darwin
AU - Siegumfeldt, Henrik
AU - Glückstad, Jesper
AU - Arneborg, Nils
N1 - Special Section on Selected Topics in Biophotonics: Photodynamic Therapy and Optical Micromanipulation for Biophotonics
PY - 2010
Y1 - 2010
N2 - The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power—i.e. pulsing—we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.
AB - The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated—i.e., 0.7 mW—the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power—i.e. pulsing—we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.
KW - Former LIFE faculty
U2 - 10.1117/1.3430731
DO - 10.1117/1.3430731
M3 - Journal article
C2 - 20799783
VL - 15
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 4
ER -
ID: 32445247