ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae
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ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae. / Bojer, Martin Saxtorph; Struve, Carsten; Ingmer, Hanne; Krogfelt, Karen Angeliki.
In: Research in Microbiology, Vol. 164, No. 3, 2013, p. 205-210.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae
AU - Bojer, Martin Saxtorph
AU - Struve, Carsten
AU - Ingmer, Hanne
AU - Krogfelt, Karen Angeliki
N1 - Copyright © 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
PY - 2013
Y1 - 2013
N2 - The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.
AB - The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.
KW - Former LIFE faculty
KW - Klebsiella pneumoniae
KW - Clp ATPase
KW - ClpK
KW - Heat resistance
KW - ClpP
KW - sHSP
U2 - 10.1016/j.resmic.2012.11.005
DO - 10.1016/j.resmic.2012.11.005
M3 - Journal article
C2 - 23201206
VL - 164
SP - 205
EP - 210
JO - Research in Microbiology
JF - Research in Microbiology
SN - 0923-2508
IS - 3
ER -
ID: 43934454