RNA preparation and characterization for gene expression studies
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RNA preparation and characterization for gene expression studies. / Stangegaard, Michael.
Methods in Molecular Biology: DNA Microarrays for Biomedical Research. red. / Hans Martin Dufva. Bind 529 1. udg. New York, NY : Springer Science+Business Media, 2009. s. 115-132.Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Undervisning
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TY - CHAP
T1 - RNA preparation and characterization for gene expression studies
AU - Stangegaard, Michael
N1 - ikke relevant
PY - 2009
Y1 - 2009
N2 - Much information can be obtained from knowledge of the relative expression level of each gene in the transcriptome. With the current advances in technology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10(6)-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization to DNA microarrays.
AB - Much information can be obtained from knowledge of the relative expression level of each gene in the transcriptome. With the current advances in technology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10(6)-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization to DNA microarrays.
KW - Faculty of Social Sciences
U2 - 10.1007/978-1-59745-538-1_8
DO - 10.1007/978-1-59745-538-1_8
M3 - Book chapter
C2 - 19381966
SN - 1064-3745
SN - 978-1-934115-69-5
VL - 529
SP - 115
EP - 132
BT - Methods in Molecular Biology
A2 - Dufva, Hans Martin
PB - Springer Science+Business Media
CY - New York, NY
ER -
ID: 13973983