Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens
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Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens. / Mbuthia, P.G.; Njagi, L.W.; Nyaga, P.N.; Bebora, L.C.; Minga, U.; Christensen, Jens Peter; Olsen, John Elmerdahl.
In: Avian Pathology, Vol. 40, No. 6, 2011, p. 629-637.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Time-course investigation of infection with a low virulent Pasteurella multocida strain in normal and immune-suppressed 12-week-old free-range chickens
AU - Mbuthia, P.G.
AU - Njagi, L.W.
AU - Nyaga, P.N.
AU - Bebora, L.C.
AU - Minga, U.
AU - Christensen, Jens Peter
AU - Olsen, John Elmerdahl
PY - 2011
Y1 - 2011
N2 - Twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (IS) or non-immune-suppressed (NIS), were challenged with a low virulent strain, Pasteurella multocida strain NCTC 10322(T), and developed clinical signs and pathological lesions typical of chronic fowl cholera. NIS birds demonstrated much more severe signs of fowl cholera than IS birds. With few exceptions, signs recorded in IS and NIS birds were of the same types, but significantly milder in the IS birds, indicating that immune suppression does not change the course of infection but rather the severity of signs in fowl cholera. P. multocida signals by fluorescent in situ hybridization (FISH) were observed between 1 h and 14 days in the lungs, trachea, air sacs, liver, spleen, bursa of Fabricius and caecal tonsils, while signals from other organs mostly were observed after 24 h. More organs had FISH signals in NIS birds than in IS birds and at higher frequency per organ. Many organs were positive by FISH even 14 days post infection, and it is suggested that these organs may be likely places for long-term carriage of P. multocida following infection. The present study has demonstrated the spread of P. multocida in different tissues in chickens and distribution of lesions associated with chronic fowl cholera, and pointed to a decrease of pathology in IS birds. Since dexamethasone mostly affects heterophils, the study suggests that these cells play a role in the development of lesions associated with chronic fowl cholera in chickens.
AB - Twelve-week-old indigenous chickens, either immune-suppressed using dexamethasone (IS) or non-immune-suppressed (NIS), were challenged with a low virulent strain, Pasteurella multocida strain NCTC 10322(T), and developed clinical signs and pathological lesions typical of chronic fowl cholera. NIS birds demonstrated much more severe signs of fowl cholera than IS birds. With few exceptions, signs recorded in IS and NIS birds were of the same types, but significantly milder in the IS birds, indicating that immune suppression does not change the course of infection but rather the severity of signs in fowl cholera. P. multocida signals by fluorescent in situ hybridization (FISH) were observed between 1 h and 14 days in the lungs, trachea, air sacs, liver, spleen, bursa of Fabricius and caecal tonsils, while signals from other organs mostly were observed after 24 h. More organs had FISH signals in NIS birds than in IS birds and at higher frequency per organ. Many organs were positive by FISH even 14 days post infection, and it is suggested that these organs may be likely places for long-term carriage of P. multocida following infection. The present study has demonstrated the spread of P. multocida in different tissues in chickens and distribution of lesions associated with chronic fowl cholera, and pointed to a decrease of pathology in IS birds. Since dexamethasone mostly affects heterophils, the study suggests that these cells play a role in the development of lesions associated with chronic fowl cholera in chickens.
KW - Analysis of Variance
KW - Animals
KW - Bacterial Load
KW - Chickens
KW - Cholera
KW - Dexamethasone
KW - Histological Techniques
KW - Immunosuppression
KW - In Situ Hybridization, Fluorescence
KW - Pasteurella Infections
KW - Pasteurella multocida
KW - Poultry Diseases
KW - Time Factors
KW - Former LIFE faculty
KW - Pasteurella
KW - multocida
KW - chicken
KW - immune supression
U2 - 10.1080/03079457.2011.623298
DO - 10.1080/03079457.2011.623298
M3 - Journal article
C2 - 22107097
VL - 40
SP - 629
EP - 637
JO - Avian Pathology
JF - Avian Pathology
SN - 0307-9457
IS - 6
ER -
ID: 38172015