Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain
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Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain. / Vognsen, Tina; Möller, Ingvar Rúnar; Kristensen, Ole.
In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online, Vol. 67, No. Pt 1, 01.01.2011, p. 48-50.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain
AU - Vognsen, Tina
AU - Möller, Ingvar Rúnar
AU - Kristensen, Ole
PY - 2011/1/1
Y1 - 2011/1/1
N2 - The nuclear transport factor 2-like (NTF2-like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging-drop vapour-diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a=b=89.84, c=70.02 Å. The crystals contained one molecule per asymmetric unit, with an estimated solvent content of 56%. Initial phases were obtained by molecular replacement.
AB - The nuclear transport factor 2-like (NTF2-like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging-drop vapour-diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a=b=89.84, c=70.02 Å. The crystals contained one molecule per asymmetric unit, with an estimated solvent content of 56%. Initial phases were obtained by molecular replacement.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1107/S1744309110042156
DO - 10.1107/S1744309110042156
M3 - Journal article
C2 - 21206022
VL - 67
SP - 48
EP - 50
JO - Acta Crystallographica Section F: Structural Biology Communications
JF - Acta Crystallographica Section F: Structural Biology Communications
SN - 2053-230X
IS - Pt 1
ER -
ID: 33812118