A simple method for validation and verification of pipettes mounted on automated liquid handlers
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A simple method for validation and verification of pipettes mounted on automated liquid handlers. / Stangegaard, Michael; Hansen, Anders Johannes; Frøslev, Tobias Guldberg; Morling, Niels.
2009. Poster session presented at LabAutomation2009, Palm Springs 2009, United States.Research output: Contribution to conference › Poster › Research
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TY - CONF
T1 - A simple method for validation and verification of pipettes mounted on automated liquid handlers
AU - Stangegaard, Michael
AU - Hansen, Anders Johannes
AU - Frøslev, Tobias Guldberg
AU - Morling, Niels
N1 - Titel på proceedings: LabAutomation2009 Sider: 134
PY - 2009
Y1 - 2009
N2 - We have implemented a simple method for validation and verification of the performance of pipettes mounted on automated liquid handlers as necessary for laboratories accredited under ISO 17025. An 8-step serial dilution of Orange G was prepared in quadruplicates in a flat bottom 96-well microtiter plate (BD Falcon) manually by means of calibrated pipettes. Each pipette of the liquid handler (1 up to 8) dispensed a selected volume (1 to 200µl) of Orange G 8 times into the wells of the microtiter plate. All wells contained a total of 200µL liquid. The optical density (OD) was read at 490 nm, and the dispensed volume of each pipette was calculated based on a plot of volume and OD of a known set of Orange G dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette was calculated. Using pre-defined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid handler software. We have implemented the method on a Sias Xantus, an MWGt TheONYX, three Tecan Freedom EVO, a Biomek NX Span-8 and four Biomek 3000 robots, and the scripts are freely available. In conclusion, we have set up a simple solution for the continuous validation of automated liquid handlers used for accredited work. The method is cheap, simple and easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.
AB - We have implemented a simple method for validation and verification of the performance of pipettes mounted on automated liquid handlers as necessary for laboratories accredited under ISO 17025. An 8-step serial dilution of Orange G was prepared in quadruplicates in a flat bottom 96-well microtiter plate (BD Falcon) manually by means of calibrated pipettes. Each pipette of the liquid handler (1 up to 8) dispensed a selected volume (1 to 200µl) of Orange G 8 times into the wells of the microtiter plate. All wells contained a total of 200µL liquid. The optical density (OD) was read at 490 nm, and the dispensed volume of each pipette was calculated based on a plot of volume and OD of a known set of Orange G dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette was calculated. Using pre-defined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid handler software. We have implemented the method on a Sias Xantus, an MWGt TheONYX, three Tecan Freedom EVO, a Biomek NX Span-8 and four Biomek 3000 robots, and the scripts are freely available. In conclusion, we have set up a simple solution for the continuous validation of automated liquid handlers used for accredited work. The method is cheap, simple and easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.
KW - Faculty of Health and Medical Sciences
M3 - Poster
Y2 - 25 January 2009 through 28 January 2009
ER -
ID: 14880669